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prism 377 dna sequencer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prism 377 dna sequencer
    Prism 377 Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+377+dna+sequencer/Deoxyribonucleic+acid/pm41594355-63-18-22
    Average 99 stars, based on 1 article reviews
    prism 377 dna sequencer - by Bioz Stars, 2026-10
    99/100 stars

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    Sequencing:

    Article Title: Association of SLC11A1 3′UTR (GT) n Microsatellite Polymorphisms with Resistance to Paratuberculosis in Sheep
    Article Snippet: .. The resulting 346 bp amplicons were sequenced on both strands using the BigDye ® Terminator Cycle Sequencing Kit and a PRISM ® 377 DNA Sequencer (Applied Biosystems (Thermo Fisher Scientific), Waltham, MA, USA). ..

    Article Title: Methods of detecting prostate cancer
    Article Snippet: Exemplary sequencing techniques include targeted sequencing, single molecule real-time sequencing, whole transcriptome shotgun sequencing (“RNA-seq”), electron microscopy-based sequencing, transistor-mediated sequencing, direct sequencing, random shotgun sequencing, Sanger dideoxy termination sequencing, exon sequencing, whole-genome sequencing, sequencing by hybridization, pyrosequencing, capillary electrophoresis, gel el ectrophoresis, duplex sequencing, cycle sequencing, single-base extension sequencing, solid-phase sequencing, high-throughput sequencing, massively parallel signature sequencing, emulsion PCR, co-amplification at lower denaturation temperature-PCR (COLD-PCR), multiplex PCR, sequencing by reversible dye terminator, paired-end sequencing, near-term sequencing, exonuclease sequencing, sequencing by ligation, short-read sequencing, single-molecule sequencing, sequencing-by-synthesis, real-time sequencing, reverse-terminator sequencing, nanopore sequencing, 454 sequencing, Solexa Genome Analyzer sequencing, SOLiD® sequencing, MS-PET sequencing, mass spectrometry, and a combination thereof. .. In some embodiments, sequencing comprises an detecting the sequencing product using an instrument, for example but not limited to an ABI PRISM® 377 DNA Sequencer, an ABI PRISM® 310, 3100, 3100-Avant, 3730, or 373OxI Genetic Analyzer, an ABI PRISM® 3700 DNA Analyzer, or an Applied Biosystems SOLiDTM System (all from Applied Biosystems), a Genome Sequencer 20 System (Roche Applied Science), or a mass spectrometer. ..

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea.
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Mass Spectrometry:

    Article Title: Methods of detecting prostate cancer
    Article Snippet: Exemplary sequencing techniques include targeted sequencing, single molecule real-time sequencing, whole transcriptome shotgun sequencing (“RNA-seq”), electron microscopy-based sequencing, transistor-mediated sequencing, direct sequencing, random shotgun sequencing, Sanger dideoxy termination sequencing, exon sequencing, whole-genome sequencing, sequencing by hybridization, pyrosequencing, capillary electrophoresis, gel el ectrophoresis, duplex sequencing, cycle sequencing, single-base extension sequencing, solid-phase sequencing, high-throughput sequencing, massively parallel signature sequencing, emulsion PCR, co-amplification at lower denaturation temperature-PCR (COLD-PCR), multiplex PCR, sequencing by reversible dye terminator, paired-end sequencing, near-term sequencing, exonuclease sequencing, sequencing by ligation, short-read sequencing, single-molecule sequencing, sequencing-by-synthesis, real-time sequencing, reverse-terminator sequencing, nanopore sequencing, 454 sequencing, Solexa Genome Analyzer sequencing, SOLiD® sequencing, MS-PET sequencing, mass spectrometry, and a combination thereof. .. In some embodiments, sequencing comprises an detecting the sequencing product using an instrument, for example but not limited to an ABI PRISM® 377 DNA Sequencer, an ABI PRISM® 310, 3100, 3100-Avant, 3730, or 373OxI Genetic Analyzer, an ABI PRISM® 3700 DNA Analyzer, or an Applied Biosystems SOLiDTM System (all from Applied Biosystems), a Genome Sequencer 20 System (Roche Applied Science), or a mass spectrometer. ..

    Polymerase Chain Reaction:

    Article Title: Improved approaches to genotyping MAOA-uVNTR polymorphisms with novel allele discovery.
    Article Snippet: .. The specific PCR bands were purified using a QIAquick® PCR purification kit (Qiagen) and the sequences were confirmed using an ABI Prism 377 DNA sequencer. .. The PCR products containing the MAOA Scientific Reports | (2025) 15:25188 7| https://doi.org/10.1038/s41598-025-10525-w gene promoter and 5’ untranslated sequences of the 2.5R, 3.3R, 3.5R, 4.5R and 5.5R MAOA-uVNTR alleles were digested with BglII and HindIII, and ligated into the pGL3-basic luciferase reporter vector (Promega).

    Article Title: Men with genetic predisposition face greater fertility challenges when exposed to electromagnetic radiation.
    Article Snippet: 1 Cytogenetics & Genomics Research Unit, Department of Zoology, University of Calcutta, Taraknath-PalitSiksha-Prangan, Ballygunge Science College Campus,35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India 2 Department of Biotechnology, School of Life Sciences, Swami Vivekananda University, Barrackpore, West Bengal, India 3 Department of Zoology, Bijoy Krishna Girls’ College (Affiliated to University of Calcutta), Howrah, West Bengal, India 4 Institute of Reproductive Medicine (IRM), HB-36/A/3 1st Cross Rd Bidhannagar, Sector III, Bidhannagar, 700106 Kolkata, West Bengal, India Abstract Purpose This study investigates the synergistic effects of genetic variants in core meiotic regulator genes—SPO11, RNF212, and SYCP3—and co-occurring exposure to electronic radiation as risk factors for azoospermia among Bengali-speaking men from West Bengal, India.. Materials and methods A total of 708 azoospermic individuals, who tested negative for Y chromosome microdeletions, and 640 healthy controls were genotyped using Sanger’s dideoxy sequencing.. Genetic variants identified included SPO11 rs3764674T > G, MN650122delA, MN708963insA, MN708964insA, MN720359T > A; RNF212 MN737491delA, rs4045481C > T; and SYCP3 rs10860779C > A, MN901901delA, and MN995822delA.

    Article Title: Improved approaches to genotyping MAOA-uVNTR polymorphisms with novel allele discovery
    Article Snippet: .. The specific PCR bands were purified using a QIAquick ® PCR purification kit (Qiagen) and the sequences were confirmed using an ABI Prism 377 DNA sequencer. .. The PCR products containing the MAOA gene promoter and 5’ untranslated sequences of the 2.5R, 3.3R, 3.5R, 4.5R and 5.5R MAOA-uVNTR alleles were digested with Bgl II and Hind III, and ligated into the pGL3-basic luciferase reporter vector (Promega).

    Purification:

    Article Title: Improved approaches to genotyping MAOA-uVNTR polymorphisms with novel allele discovery.
    Article Snippet: .. The specific PCR bands were purified using a QIAquick® PCR purification kit (Qiagen) and the sequences were confirmed using an ABI Prism 377 DNA sequencer. .. The PCR products containing the MAOA Scientific Reports | (2025) 15:25188 7| https://doi.org/10.1038/s41598-025-10525-w gene promoter and 5’ untranslated sequences of the 2.5R, 3.3R, 3.5R, 4.5R and 5.5R MAOA-uVNTR alleles were digested with BglII and HindIII, and ligated into the pGL3-basic luciferase reporter vector (Promega).

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea.
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Article Title: Improved approaches to genotyping MAOA-uVNTR polymorphisms with novel allele discovery
    Article Snippet: .. The specific PCR bands were purified using a QIAquick ® PCR purification kit (Qiagen) and the sequences were confirmed using an ABI Prism 377 DNA sequencer. .. The PCR products containing the MAOA gene promoter and 5’ untranslated sequences of the 2.5R, 3.3R, 3.5R, 4.5R and 5.5R MAOA-uVNTR alleles were digested with Bgl II and Hind III, and ligated into the pGL3-basic luciferase reporter vector (Promega).

    Cell Culture:

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea.
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Clone Assay:

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Article Title: Morphological, Molecular and Phylogenetic Characterization of Ceratomyxa nemiptera sp. nov. (Myxozoa: Ceratomyxidae) Infecting Nemipterus virgatus Houttuyn, 1782 in the East China Sea.
    Article Snippet: .. Eight positive transformants were cultured, plasmids were purified, and four clones were selected for sequencing using an ABI Prism 377 DNA Sequencer (Applied Biosystems, Carlsbad, CA, USA). .. The resulting SSU rDNA sequences of myxospores were assembled using ContigExpress software (Vector NTI Suite 6.0, Invitrogen, Carlsbad, CA, USA), and the consensus sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank database for public access.

    Software:

    Article Title: Men with genetic predisposition face greater fertility challenges when exposed to electromagnetic radiation.
    Article Snippet: 1 Cytogenetics & Genomics Research Unit, Department of Zoology, University of Calcutta, Taraknath-PalitSiksha-Prangan, Ballygunge Science College Campus,35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India 2 Department of Biotechnology, School of Life Sciences, Swami Vivekananda University, Barrackpore, West Bengal, India 3 Department of Zoology, Bijoy Krishna Girls’ College (Affiliated to University of Calcutta), Howrah, West Bengal, India 4 Institute of Reproductive Medicine (IRM), HB-36/A/3 1st Cross Rd Bidhannagar, Sector III, Bidhannagar, 700106 Kolkata, West Bengal, India Abstract Purpose This study investigates the synergistic effects of genetic variants in core meiotic regulator genes—SPO11, RNF212, and SYCP3—and co-occurring exposure to electronic radiation as risk factors for azoospermia among Bengali-speaking men from West Bengal, India.. Materials and methods A total of 708 azoospermic individuals, who tested negative for Y chromosome microdeletions, and 640 healthy controls were genotyped using Sanger’s dideoxy sequencing.. Genetic variants identified included SPO11 rs3764674T > G, MN650122delA, MN708963insA, MN708964insA, MN720359T > A; RNF212 MN737491delA, rs4045481C > T; and SYCP3 rs10860779C > A, MN901901delA, and MN995822delA.



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